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pcdna3 1 flag nrf2 wt  (Addgene inc)


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    Structured Review

    Addgene inc pcdna3 1 flag nrf2 wt
    Pcdna3 1 Flag Nrf2 Wt, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcdna3+1+flag+nrf2+wt/NC16+pCDNA3%2E1+FLAG+NRF2+(Plasmid+%2336971)/pmc12669649-196-7-10
    Average 93 stars, based on 44 article reviews
    pcdna3 1 flag nrf2 wt - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Construct:

    Article Title: NRF2 connects Src tyrosine kinase to ferroptosis resistance in glioblastoma
    Article Snippet: For transient transfection experiments, cells were seeded the day before and transfected using polyethylenimine (PEI) (Tebu-Bio), following the manufacturer’s instructions. .. Src constructs pSGT-Src-Y527F and pSGT-K295M and empty pSGT vector were previously described ( ). pCDNA3.1-FLAG-NRF2 WT was obtained from Addgene. .. Primary antibodies used are as follows: anti-NRF2 (D1Z9C) (Cell Signaling Technology), anti-p62 (SQSTM1) (PM045; MBL International), anti-phospho-p62 (SQSTM1) (Ser349) (PM074; MBL International), anti-vinculin (E1E9V) (Cell Signaling Technology), anti-lamin (E-1) (sc-376248; Santa Cruz Biotechnology), anti-GAPDH (D16H11) (Cell Signaling Technology); anti-Src (2108S) (Cell Signaling Technology), anti-phospho-Src (Tyr416) (2101S) (Cell Signaling Technology), anti-KEAP1 (G-2) (sc-365626; Santa Cruz Biotechnology), anti-heme oxygenase 1 (A-3) (sc-136960; Santa Cruz Biotechnology), anti-NQO1 (A-180) (sc-32793; Santa Cruz Biotechnology), anti-p70S6K (Cell Signaling Technology), anti-phospho-p70S6K (Thr389) (Cell Signaling Technology), anti-ERK 1/2 (137F5) (Cell Signaling Technology), anti-phospho-ERK 1/2 (Thr202/Tyr204) (D13.14.4E) (Cell Signaling Technology), anti-ubiquitin (P4D1; Santa Cruz Biotechnology).

    Plasmid Preparation:

    Article Title: NRF2 connects Src tyrosine kinase to ferroptosis resistance in glioblastoma
    Article Snippet: For transient transfection experiments, cells were seeded the day before and transfected using polyethylenimine (PEI) (Tebu-Bio), following the manufacturer’s instructions. .. Src constructs pSGT-Src-Y527F and pSGT-K295M and empty pSGT vector were previously described ( ). pCDNA3.1-FLAG-NRF2 WT was obtained from Addgene. .. Primary antibodies used are as follows: anti-NRF2 (D1Z9C) (Cell Signaling Technology), anti-p62 (SQSTM1) (PM045; MBL International), anti-phospho-p62 (SQSTM1) (Ser349) (PM074; MBL International), anti-vinculin (E1E9V) (Cell Signaling Technology), anti-lamin (E-1) (sc-376248; Santa Cruz Biotechnology), anti-GAPDH (D16H11) (Cell Signaling Technology); anti-Src (2108S) (Cell Signaling Technology), anti-phospho-Src (Tyr416) (2101S) (Cell Signaling Technology), anti-KEAP1 (G-2) (sc-365626; Santa Cruz Biotechnology), anti-heme oxygenase 1 (A-3) (sc-136960; Santa Cruz Biotechnology), anti-NQO1 (A-180) (sc-32793; Santa Cruz Biotechnology), anti-p70S6K (Cell Signaling Technology), anti-phospho-p70S6K (Thr389) (Cell Signaling Technology), anti-ERK 1/2 (137F5) (Cell Signaling Technology), anti-phospho-ERK 1/2 (Thr202/Tyr204) (D13.14.4E) (Cell Signaling Technology), anti-ubiquitin (P4D1; Santa Cruz Biotechnology).

    Stable Transfection:

    Article Title: Caspase-8 expression and its Src dependent phosphorylation on Tyrosine 380 triggers NRF2 signaling activation in glioblastoma
    Article Snippet: .. Stable cell lines U87shC8#2 and U251shC8#2, overexpressing pCDNA3.1-FLAG-NRF2 WT (#36971, Addgene), were generated by transfection using polyethylenimine (PEI) (Tebu-Bio), following the manufacturer’s instructions. .. Cells were maintained and selected with 50 μg/ml Zeocin (Thermo Fisher) for 2 weeks.

    Generated:

    Article Title: Caspase-8 expression and its Src dependent phosphorylation on Tyrosine 380 triggers NRF2 signaling activation in glioblastoma
    Article Snippet: .. Stable cell lines U87shC8#2 and U251shC8#2, overexpressing pCDNA3.1-FLAG-NRF2 WT (#36971, Addgene), were generated by transfection using polyethylenimine (PEI) (Tebu-Bio), following the manufacturer’s instructions. .. Cells were maintained and selected with 50 μg/ml Zeocin (Thermo Fisher) for 2 weeks.

    Transfection:

    Article Title: Caspase-8 expression and its Src dependent phosphorylation on Tyrosine 380 triggers NRF2 signaling activation in glioblastoma
    Article Snippet: .. Stable cell lines U87shC8#2 and U251shC8#2, overexpressing pCDNA3.1-FLAG-NRF2 WT (#36971, Addgene), were generated by transfection using polyethylenimine (PEI) (Tebu-Bio), following the manufacturer’s instructions. .. Cells were maintained and selected with 50 μg/ml Zeocin (Thermo Fisher) for 2 weeks.



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    Addgene inc pcdna3 1 flag nrf2 wt
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